Abstract Widespread distribution of porcine epidemic diarrhea virus (PEDV) mexican tamarind candy spoon has led to catastrophic losses to the global pig farming industry.As a result, there is an urgent need for rapid, sensitive and accurate tests for PEDV to enable timely and effective interventions.In the present study, we develop and validate a floating gate carbon nanotubes field-effect transistor (FG CNT-FET)-based portable immunosensor for rapid identification of PEDV in a sensitive and accurate manner.To improve the affinity, a unique PEDV spike protein-specific monoclonal antibody is prepared by purification, and subsequently modified on FG CNT-FET sensor to recognize PEDV.The developed FET biosensor enables highly sensitive detection (LoD: 8.
1 fg/mL and 100.14 TCID50/mL for recombinant spike proteins and PEDV, respectively), as well as satisfactory specificity.Notably, an integrated portable platform consisting of a pluggable FG CNT-FET chip and a portable device can discriminate PEDV positive from negative samples and even identify PEDV and porcine deltacoronavirus within 1 min with 100% accuracy.The portable sensing platform offers sapatilha infantil prata glitter the capability to quickly, sensitively and accurately identify PEDV, which further points to a possibility of point of care (POC) applications of large-scale surveillance in pig breeding facilities.